Sulfhydryl-specific PEGylation of phosphotriesterase cysteine mutants for organophosphate detoxification
نویسندگان
چکیده
منابع مشابه
Detoxification of organophosphate nerve agents by bacterial phosphotriesterase.
Organophosphates have been widely used as insecticides and chemical warfare agents. The health risks associated with these agents have necessitated the need for better detoxification and bioremediation tools. Bacterial enzymes capable of hydrolyzing the lethal organophosphate nerve agents are of special interest. Phosphotriesterase (PTE) isolated from the soil bacteria Pseudomonas diminuta disp...
متن کاملDetoxification of organophosphate pesticides using a nylon based immobilized phosphotriesterase from Pseudomonas diminuta.
A partially purified phophotriesterase was successfully immobilized onto nylon 6 and 66 membranes, nylon 11 powder, and nylon tubing. Up to 9000 U of enzyme activity was immobilized onto 2000 cm2 of a nylon 6 membrane where 1 U is the amount of enzyme necessary to catalyze the hydrolysis of 1.0 mumol of paraoxon/min at 25 degrees C. The nylon 66 membrane-bound phosphotriesterase was characteriz...
متن کاملin silico designing of a new cysteine analogue of hirudin variant 3 for site specific pegylation
hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. recombinant hirudin (r-hir) displays certain drawbacks including bleeding and immunogenicity. to solve these problems, cysteine-specific pegylation has been proposed as a successful technique. however, proper selection of the appropriate cysteine residue for substitution is a critical step. this study has, for the ...
متن کاملIn silico designing of a new cysteine analogue of hirudin variant 3 for site specific PEGylation
Hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. Recombinant hirudin (r-Hir) displays certain drawbacks including bleeding and immunogenicity. To solve these problems, cysteine-specific PEGylation has been proposed as a successful technique. However, proper selection of the appropriate cysteine residue for substitution is a critical step. This study has, for the ...
متن کاملBacterial detoxification of organophosphate nerve agents.
Bacterial enzymes have been isolated that catalyze the hydrolysis of organophosphate nerve agents with high-rate enhancements and broad substrate specificity. Mutant forms of these enzymes have been constructed through rational redesign of the active-site binding pockets and random mutagenesis to create protein variants that are optimized for the detoxification of agricultural insecticides and ...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2015
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzv036